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fast red counterstaining  (Vector Laboratories)


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    Vector Laboratories fast red counterstaining
    Assessment of placental morphology in control, hypothyroid, and Kisspeptin‐10 (Kp10)‐treated hypothyroid rats. (A) Photomicrographs of placentas at GD 14 (Hematoxylin & Eosin; Bar = 2.5 mm). (B) Absolute volume of the placental zones at GD 14 and 18. (C) Photomicrographs of PAS staining in the junctional zone (PAS; Fast Green; Bar = 50 μm). (D) Area in pixels stained by PAS at GD 14 and 18. (E) Photomicrographs of the double staining by cytokeratin + vimentin (Streptavidin‐biotin‐peroxidase; Alkaline Phosphatase‐BCIP/NBT; nuclear Fast Red; Bar = 20 μm) (F) Absolute volume of the compartments of the labyrinth zone at GD 14 and 18. (G) Surface area of the vasculature of the labyrinth area at GD 14 and 18. (H) Total fetal capillary length at GD 14 and 18. (I) Fetal capillary area at GD 14 and 18. (J) Fetal capillary diameter at GD 14 and 18. (K) Interhaemal membrane thickness at GD 14 and 18. (L) Theoretical diffusion capacity of the interhaemal membrane at GD 14 and 18. (M) Specific diffusion capacity of the interhaemal membrane at GD 14 and 18. (Mean ± SEM). Significant differences are indicated by * p < 0.05, ** p < 0.01 for one‐way ANOVA followed by SNK test, and # p < 0.05 for Student's t ‐test. BD, basal decidua; GD, gestational day; Gly, glycogen cells; Green bar, interhaemal membraneJZ, junctional zone; LZ, labyrinth zone; MVS, maternal vascular space; PAS, Periodic acid‐Schiff; SpT, spongiotrophoblast; TB, trophoblast; TGC, trophoblast giant cells.
    Fast Red Counterstaining, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1027 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 96 stars, based on 1027 article reviews
    fast red counterstaining - by Bioz Stars, 2026-04
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    Images

    1) Product Images from "Kisspeptin Restores Placental mTOR Signaling and Improves Glucose Homeostasis Mediators Disrupted by Maternal Hypothyroidism in Rats"

    Article Title: Kisspeptin Restores Placental mTOR Signaling and Improves Glucose Homeostasis Mediators Disrupted by Maternal Hypothyroidism in Rats

    Journal: Acta Physiologica (Oxford, England)

    doi: 10.1111/apha.70188

    Assessment of placental morphology in control, hypothyroid, and Kisspeptin‐10 (Kp10)‐treated hypothyroid rats. (A) Photomicrographs of placentas at GD 14 (Hematoxylin & Eosin; Bar = 2.5 mm). (B) Absolute volume of the placental zones at GD 14 and 18. (C) Photomicrographs of PAS staining in the junctional zone (PAS; Fast Green; Bar = 50 μm). (D) Area in pixels stained by PAS at GD 14 and 18. (E) Photomicrographs of the double staining by cytokeratin + vimentin (Streptavidin‐biotin‐peroxidase; Alkaline Phosphatase‐BCIP/NBT; nuclear Fast Red; Bar = 20 μm) (F) Absolute volume of the compartments of the labyrinth zone at GD 14 and 18. (G) Surface area of the vasculature of the labyrinth area at GD 14 and 18. (H) Total fetal capillary length at GD 14 and 18. (I) Fetal capillary area at GD 14 and 18. (J) Fetal capillary diameter at GD 14 and 18. (K) Interhaemal membrane thickness at GD 14 and 18. (L) Theoretical diffusion capacity of the interhaemal membrane at GD 14 and 18. (M) Specific diffusion capacity of the interhaemal membrane at GD 14 and 18. (Mean ± SEM). Significant differences are indicated by * p < 0.05, ** p < 0.01 for one‐way ANOVA followed by SNK test, and # p < 0.05 for Student's t ‐test. BD, basal decidua; GD, gestational day; Gly, glycogen cells; Green bar, interhaemal membraneJZ, junctional zone; LZ, labyrinth zone; MVS, maternal vascular space; PAS, Periodic acid‐Schiff; SpT, spongiotrophoblast; TB, trophoblast; TGC, trophoblast giant cells.
    Figure Legend Snippet: Assessment of placental morphology in control, hypothyroid, and Kisspeptin‐10 (Kp10)‐treated hypothyroid rats. (A) Photomicrographs of placentas at GD 14 (Hematoxylin & Eosin; Bar = 2.5 mm). (B) Absolute volume of the placental zones at GD 14 and 18. (C) Photomicrographs of PAS staining in the junctional zone (PAS; Fast Green; Bar = 50 μm). (D) Area in pixels stained by PAS at GD 14 and 18. (E) Photomicrographs of the double staining by cytokeratin + vimentin (Streptavidin‐biotin‐peroxidase; Alkaline Phosphatase‐BCIP/NBT; nuclear Fast Red; Bar = 20 μm) (F) Absolute volume of the compartments of the labyrinth zone at GD 14 and 18. (G) Surface area of the vasculature of the labyrinth area at GD 14 and 18. (H) Total fetal capillary length at GD 14 and 18. (I) Fetal capillary area at GD 14 and 18. (J) Fetal capillary diameter at GD 14 and 18. (K) Interhaemal membrane thickness at GD 14 and 18. (L) Theoretical diffusion capacity of the interhaemal membrane at GD 14 and 18. (M) Specific diffusion capacity of the interhaemal membrane at GD 14 and 18. (Mean ± SEM). Significant differences are indicated by * p < 0.05, ** p < 0.01 for one‐way ANOVA followed by SNK test, and # p < 0.05 for Student's t ‐test. BD, basal decidua; GD, gestational day; Gly, glycogen cells; Green bar, interhaemal membraneJZ, junctional zone; LZ, labyrinth zone; MVS, maternal vascular space; PAS, Periodic acid‐Schiff; SpT, spongiotrophoblast; TB, trophoblast; TGC, trophoblast giant cells.

    Techniques Used: Control, Staining, Double Staining, Membrane, Diffusion-based Assay



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    Assessment of placental morphology in control, hypothyroid, and Kisspeptin‐10 (Kp10)‐treated hypothyroid rats. (A) Photomicrographs of placentas at GD 14 (Hematoxylin & Eosin; Bar = 2.5 mm). (B) Absolute volume of the placental zones at GD 14 and 18. (C) Photomicrographs of PAS staining in the junctional zone (PAS; Fast Green; Bar = 50 μm). (D) Area in pixels stained by PAS at GD 14 and 18. (E) Photomicrographs of the double staining by cytokeratin + vimentin (Streptavidin‐biotin‐peroxidase; Alkaline Phosphatase‐BCIP/NBT; nuclear Fast Red; Bar = 20 μm) (F) Absolute volume of the compartments of the labyrinth zone at GD 14 and 18. (G) Surface area of the vasculature of the labyrinth area at GD 14 and 18. (H) Total fetal capillary length at GD 14 and 18. (I) Fetal capillary area at GD 14 and 18. (J) Fetal capillary diameter at GD 14 and 18. (K) Interhaemal membrane thickness at GD 14 and 18. (L) Theoretical diffusion capacity of the interhaemal membrane at GD 14 and 18. (M) Specific diffusion capacity of the interhaemal membrane at GD 14 and 18. (Mean ± SEM). Significant differences are indicated by * p < 0.05, ** p < 0.01 for one‐way ANOVA followed by SNK test, and # p < 0.05 for Student's t ‐test. BD, basal decidua; GD, gestational day; Gly, glycogen cells; Green bar, interhaemal membraneJZ, junctional zone; LZ, labyrinth zone; MVS, maternal vascular space; PAS, Periodic acid‐Schiff; SpT, spongiotrophoblast; TB, trophoblast; TGC, trophoblast giant cells.
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    Assessment of placental morphology in control, hypothyroid, and Kisspeptin‐10 (Kp10)‐treated hypothyroid rats. (A) Photomicrographs of placentas at GD 14 (Hematoxylin & Eosin; Bar = 2.5 mm). (B) Absolute volume of the placental zones at GD 14 and 18. (C) Photomicrographs of PAS staining in the junctional zone (PAS; Fast Green; Bar = 50 μm). (D) Area in pixels stained by PAS at GD 14 and 18. (E) Photomicrographs of the double staining by cytokeratin + vimentin (Streptavidin‐biotin‐peroxidase; Alkaline Phosphatase‐BCIP/NBT; nuclear Fast Red; Bar = 20 μm) (F) Absolute volume of the compartments of the labyrinth zone at GD 14 and 18. (G) Surface area of the vasculature of the labyrinth area at GD 14 and 18. (H) Total fetal capillary length at GD 14 and 18. (I) Fetal capillary area at GD 14 and 18. (J) Fetal capillary diameter at GD 14 and 18. (K) Interhaemal membrane thickness at GD 14 and 18. (L) Theoretical diffusion capacity of the interhaemal membrane at GD 14 and 18. (M) Specific diffusion capacity of the interhaemal membrane at GD 14 and 18. (Mean ± SEM). Significant differences are indicated by * p < 0.05, ** p < 0.01 for one‐way ANOVA followed by SNK test, and # p < 0.05 for Student's t ‐test. BD, basal decidua; GD, gestational day; Gly, glycogen cells; Green bar, interhaemal membraneJZ, junctional zone; LZ, labyrinth zone; MVS, maternal vascular space; PAS, Periodic acid‐Schiff; SpT, spongiotrophoblast; TB, trophoblast; TGC, trophoblast giant cells.
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    Assessment of placental morphology in control, hypothyroid, and Kisspeptin‐10 (Kp10)‐treated hypothyroid rats. (A) Photomicrographs of placentas at GD 14 (Hematoxylin & Eosin; Bar = 2.5 mm). (B) Absolute volume of the placental zones at GD 14 and 18. (C) Photomicrographs of PAS staining in the junctional zone (PAS; Fast Green; Bar = 50 μm). (D) Area in pixels stained by PAS at GD 14 and 18. (E) Photomicrographs of the double staining by cytokeratin + vimentin (Streptavidin‐biotin‐peroxidase; Alkaline Phosphatase‐BCIP/NBT; nuclear Fast Red; Bar = 20 μm) (F) Absolute volume of the compartments of the labyrinth zone at GD 14 and 18. (G) Surface area of the vasculature of the labyrinth area at GD 14 and 18. (H) Total fetal capillary length at GD 14 and 18. (I) Fetal capillary area at GD 14 and 18. (J) Fetal capillary diameter at GD 14 and 18. (K) Interhaemal membrane thickness at GD 14 and 18. (L) Theoretical diffusion capacity of the interhaemal membrane at GD 14 and 18. (M) Specific diffusion capacity of the interhaemal membrane at GD 14 and 18. (Mean ± SEM). Significant differences are indicated by * p < 0.05, ** p < 0.01 for one‐way ANOVA followed by SNK test, and # p < 0.05 for Student's t ‐test. BD, basal decidua; GD, gestational day; Gly, glycogen cells; Green bar, interhaemal membraneJZ, junctional zone; LZ, labyrinth zone; MVS, maternal vascular space; PAS, Periodic acid‐Schiff; SpT, spongiotrophoblast; TB, trophoblast; TGC, trophoblast giant cells.
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    Assessment of placental morphology in control, hypothyroid, and Kisspeptin‐10 (Kp10)‐treated hypothyroid rats. (A) Photomicrographs of placentas at GD 14 (Hematoxylin & Eosin; Bar = 2.5 mm). (B) Absolute volume of the placental zones at GD 14 and 18. (C) Photomicrographs of PAS staining in the junctional zone (PAS; Fast Green; Bar = 50 μm). (D) Area in pixels stained by PAS at GD 14 and 18. (E) Photomicrographs of the double staining by cytokeratin + vimentin (Streptavidin‐biotin‐peroxidase; Alkaline Phosphatase‐BCIP/NBT; nuclear Fast Red; Bar = 20 μm) (F) Absolute volume of the compartments of the labyrinth zone at GD 14 and 18. (G) Surface area of the vasculature of the labyrinth area at GD 14 and 18. (H) Total fetal capillary length at GD 14 and 18. (I) Fetal capillary area at GD 14 and 18. (J) Fetal capillary diameter at GD 14 and 18. (K) Interhaemal membrane thickness at GD 14 and 18. (L) Theoretical diffusion capacity of the interhaemal membrane at GD 14 and 18. (M) Specific diffusion capacity of the interhaemal membrane at GD 14 and 18. (Mean ± SEM). Significant differences are indicated by * p < 0.05, ** p < 0.01 for one‐way ANOVA followed by SNK test, and # p < 0.05 for Student's t ‐test. BD, basal decidua; GD, gestational day; Gly, glycogen cells; Green bar, interhaemal membraneJZ, junctional zone; LZ, labyrinth zone; MVS, maternal vascular space; PAS, Periodic acid‐Schiff; SpT, spongiotrophoblast; TB, trophoblast; TGC, trophoblast giant cells.
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    Image Search Results


    Assessment of placental morphology in control, hypothyroid, and Kisspeptin‐10 (Kp10)‐treated hypothyroid rats. (A) Photomicrographs of placentas at GD 14 (Hematoxylin & Eosin; Bar = 2.5 mm). (B) Absolute volume of the placental zones at GD 14 and 18. (C) Photomicrographs of PAS staining in the junctional zone (PAS; Fast Green; Bar = 50 μm). (D) Area in pixels stained by PAS at GD 14 and 18. (E) Photomicrographs of the double staining by cytokeratin + vimentin (Streptavidin‐biotin‐peroxidase; Alkaline Phosphatase‐BCIP/NBT; nuclear Fast Red; Bar = 20 μm) (F) Absolute volume of the compartments of the labyrinth zone at GD 14 and 18. (G) Surface area of the vasculature of the labyrinth area at GD 14 and 18. (H) Total fetal capillary length at GD 14 and 18. (I) Fetal capillary area at GD 14 and 18. (J) Fetal capillary diameter at GD 14 and 18. (K) Interhaemal membrane thickness at GD 14 and 18. (L) Theoretical diffusion capacity of the interhaemal membrane at GD 14 and 18. (M) Specific diffusion capacity of the interhaemal membrane at GD 14 and 18. (Mean ± SEM). Significant differences are indicated by * p < 0.05, ** p < 0.01 for one‐way ANOVA followed by SNK test, and # p < 0.05 for Student's t ‐test. BD, basal decidua; GD, gestational day; Gly, glycogen cells; Green bar, interhaemal membraneJZ, junctional zone; LZ, labyrinth zone; MVS, maternal vascular space; PAS, Periodic acid‐Schiff; SpT, spongiotrophoblast; TB, trophoblast; TGC, trophoblast giant cells.

    Journal: Acta Physiologica (Oxford, England)

    Article Title: Kisspeptin Restores Placental mTOR Signaling and Improves Glucose Homeostasis Mediators Disrupted by Maternal Hypothyroidism in Rats

    doi: 10.1111/apha.70188

    Figure Lengend Snippet: Assessment of placental morphology in control, hypothyroid, and Kisspeptin‐10 (Kp10)‐treated hypothyroid rats. (A) Photomicrographs of placentas at GD 14 (Hematoxylin & Eosin; Bar = 2.5 mm). (B) Absolute volume of the placental zones at GD 14 and 18. (C) Photomicrographs of PAS staining in the junctional zone (PAS; Fast Green; Bar = 50 μm). (D) Area in pixels stained by PAS at GD 14 and 18. (E) Photomicrographs of the double staining by cytokeratin + vimentin (Streptavidin‐biotin‐peroxidase; Alkaline Phosphatase‐BCIP/NBT; nuclear Fast Red; Bar = 20 μm) (F) Absolute volume of the compartments of the labyrinth zone at GD 14 and 18. (G) Surface area of the vasculature of the labyrinth area at GD 14 and 18. (H) Total fetal capillary length at GD 14 and 18. (I) Fetal capillary area at GD 14 and 18. (J) Fetal capillary diameter at GD 14 and 18. (K) Interhaemal membrane thickness at GD 14 and 18. (L) Theoretical diffusion capacity of the interhaemal membrane at GD 14 and 18. (M) Specific diffusion capacity of the interhaemal membrane at GD 14 and 18. (Mean ± SEM). Significant differences are indicated by * p < 0.05, ** p < 0.01 for one‐way ANOVA followed by SNK test, and # p < 0.05 for Student's t ‐test. BD, basal decidua; GD, gestational day; Gly, glycogen cells; Green bar, interhaemal membraneJZ, junctional zone; LZ, labyrinth zone; MVS, maternal vascular space; PAS, Periodic acid‐Schiff; SpT, spongiotrophoblast; TB, trophoblast; TGC, trophoblast giant cells.

    Article Snippet: The next day, sections were kept at room temperature (2 h), and incubated with alkaline phosphatase–conjugated secondary antibody (ab6722, 1:500, Abcam, 1 h) and developed with NBT/BCIP (34 042, Thermo Scientific, 10 min) followed by Fast Red counterstaining (H‐3403, Vector Laboratories, 4 min).

    Techniques: Control, Staining, Double Staining, Membrane, Diffusion-based Assay